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R&D Systems recombinant mouse mcsf
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Recombinant Mouse Mcsf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant mouse mcsf - by Bioz Stars, 2026-03
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95
Bio-Techne corporation recombinant mouse trance/rank l/tnfsf11 (e. coli-expressed)
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Recombinant Mouse Trance/Rank L/Tnfsf11 (E. Coli Expressed), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant mouse icam-1/cd54 fc chimera protein, cf
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Recombinant Mouse Icam 1/Cd54 Fc Chimera Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant mouse jam-a fc chimera protein, cf
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Recombinant Mouse Jam A Fc Chimera Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation bms 493
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Bms 493, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant mouse dner fc chimera protein, cf
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Recombinant Mouse Dner Fc Chimera Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant mouse m-csf protein, cf
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Recombinant Mouse M Csf Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant human dll3 protein, cf
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
Recombinant Human Dll3 Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation retinoic acid
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
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Greiner Bio nontreated tissue culture petri dishes
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
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Cayman Chemical npd1 cayman chemical 10 010 390
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
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ibidi GmbH 8-well μ-slide imaging slides cat. no. 80826
a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with <t>MCSF</t> or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.
8 Well μ Slide Imaging Slides Cat. No. 80826, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with MCSF or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Brain milieu induces early microglial maturation through the BAX-Notch axis

doi: 10.1038/s41467-022-33836-2

Figure Lengend Snippet: a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with MCSF or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.

Article Snippet: Sorted CD11b + cells were cultured at 24-well plate (6 × 10 4 cells per well) coated with poly-D-lysine, and grown in microglia culture medium containing 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, 10 ng/ml recombinant mouse MCSF (R&D Systems, 416-ML-010/CF) with or without the presence of 1 μg/ml recombinant human DLL3 (R&D Systems, 9749-DL-050) for 5 days.

Techniques: Immunofluorescence, In Vitro, Cell Culture, Activation Assay, Two Tailed Test